Collagenase NB 6 GMP grade by Serva/Nordmark
Clostridiopeptidase A, MW 70kDa - 120kDa, CAS 9001-12-1
Collagenase NB 6 cGMP is a sterile product made to EU GMP guidelines for applications that must meet collagenase cGMP standards for cell isolation applications in tissue engineering and tissue transplantation. Collagenase NB 6 GMP is used in tissue dissociation and cell isolation for human adult stem cells, chondocytes, fibroblasts, neronal ad endothelial cell types.
This collagenase produced from Clostridium histolyticum. It is chromatographically purified and contains class I and class II collagenases with balanced proteolytic activities.
Applications for Collagenase NB 6 cGMP includes cell isolation of:
- Islets of Langerhans
- Chromaffin cells
- Endothelial cells
- Epithelial cells
Collagenase NB 6 cGMP activitie(s)
- PZ (Wunsch): greater than 0.100 U/mg
- Endotoxin: less than 100 EU/mg (Ph.Eur.)
Unit definition: 1U catalyzes the hydrolysis of 1umole 4-phenylazobenzyloxycarbonyl-L-prolyl-D-arginine per minute at 25C.
Store between 2-8C or 35 - 46F
Image credit: By Deposition authors: Spurlino, J.C., Smith, D.L.;visualization author: User:Astrojan - http://www.rcsb.org/pdb/explore/explore.do?structureId=1hfc, CC BY 3.0, Link