Collagenase NB 4 Standard grade by Serva/Nordmark
Clostridiopeptidase A, MW 70kDa - 120kDa, CAS 9001-12-1
Collagenase NB 4 Standard grade is made according to EU GMP guidelines for cell isolation applications in tissue engineering and tissue transplantation. This collagenase has similar activity as Collagenase NB 6 and Collagenase NB 5. Collagenase NB 4 can be used in cell isolation for human, rodent and frog cell types.
This collagenase produced from Clostridium histolyticum. It is chromatographically purified and contains class I and class II collagenases with balanced mix of Trypsin like activity, neutral protease and clostripain.
Applications for Collagenase NB 4 standard includes cell isolation of:
- Human hepatocytes
- Human Chondrocytes
- Human endometrial cells
- Human endothelial cells (umbilical cord, foreskin)
- Frog oocytes
- Bovine and rat Chromaffin cells
- Osteoblasts (human, mouse)
- Stem cells (Whartons jelly, lipoaspirate)
- Tumor cells (Colon)
Collagenase NB 4 sterile activitie(s)
- PZ (Wunsch): greater than 0.10 U/mg
Unit definition: 1U catalyzes the hydrolysis of 1umole 4-phenylazobenzyloxycarbonyl-L-prolyl-D-arginine per minute at 25C.
Store between 2-8C or 35 - 46F
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