Collagenase NB 1 Premium grade by Serva/Nordmark
Clostridiopeptidase A, MW 70kDa - 120kDa, CAS 9001-12-1
Collagenase NB 1 Premium grade is for research into cell isolation applications in tissue engineering and tissue transplantation. This collagenase is highly purified to provide high yields and viability of very sensitive cells. Collagenase NB 1 Premium is especially applicable to isolation of pancreatic Islets of Langerhans cells across many species. It is suggested to use with Neutral Protease NB GMP Grade (ref. no. 30303). If isolated cells are intended for use with human transplantation please use Collagenase NB 1 GMP (ref. no. 17452)
This collagenase is produced from Clostridium histolyticum. It is chromatographically purified and contains class I and class II collagenases mostly free of clostripain, trypsin-like and neutral protease activity.
Applications for Collagenase NB 1 Premium includes cell isolation of:
- Human pancreatic islets of Langerhans
- Porcine islets
Collagenase NB 1 Premium activitie(s)
- PZ (Wunsch): greater than 0.30 U/mg
- Endotoxin: less than 10.0 EU/mg (Ph. Eur.)
Each vial contains not less than 2000 PZ U of collagenase
Unit definition: 1U catalyzes the hydrolysis of 1umole 4-phenylazobenzyloxycarbonyl-L-prolyl-D-arginine per minute at 25C.
Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE))
Store between 2-8C or 35 - 46F
Image credit: By Deposition authors: Spurlino, J.C., Smith, D.L.;visualization author: User:Astrojan - http://www.rcsb.org/pdb/explore/explore.do?structureId=1hfc, CC BY 3.0, Link