Lactate dehydrogenase from porcine heart min. 300 U/mg
Lactate dyhrogenase (LDH) is an enzyme found throughout living tissues and is used for cellular energy production; catalyzing NAD+ to NADH and the reverse reaction. It can be used in the determination of lactate and in coupled systems for detection of biological metabolites.
This formulation of Lactate dehydrogenase is a highly purified suspension of 2 x crystalized in 70% saturated ammonium sulphate solultion.
Activity: 300 U/mg protein
Mw: 140 000
Preparation contains predominantly isoenzymes LDH 1 and 2. LDH is used for the
Extraneous activities: PK, GPT, each max. 0.01 %; MDH max. 0.05 %
Unit definition: 1 U catalyzes the reduction of 1 µmole of pyruvate to L-lactate per minute at 25 °C, pH 7.0 in the presence of NADH (oxidationof 1 µmole NADH).
Storage Temperature: +2 °C to +8 °C
A QUALITY SERVA ELECTROPHORESIS PRODUCT